Review

recombinant mouse ephb2 protein (MedChemExpress)

MedChemExpress is a verified supplier

MedChemExpress manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

MedChemExpress recombinant mouse ephb2 protein

The activation of <t>EFNB1/EphB2</t> interaction in dual-task intervention is regulated by miR-204. a Immunofluorescence of EFNB1 (red), EphB2 (green), NeuN (pink), with DAPI (blue). Scale bar 20 μm (Merge). b Quantification of EFNB1/EphB2 co-expression fluorescence intensity. F (4, 10) = 13.35, P < 0.001. c Expression of EFNB1 and EphB2 in the hippocampus detected by WB. d , e Quantification of EFNB1 and EphB2 expression in the hippocampus. EFNB1: H = 23.82, P < 0.001. EphB2: F (3, 26) = 18.69, P < 0.001. f EFNB1/EphB2 interactions in the hippocampus detected by co-IP. g Expression of EFNB1 and EphB2 in HT22 cells after silencing or overexpression lncRNA NEAT1/miR-204 detected by WB. h , i Quantification of EFNB1 and EphB2 expression in cells. EFNB1: F (4, 10) = 22.69, P < 0.001; EphB2: F (4, 10) = 18.13, P < 0.001 j EFNB1/EphB2 interactions in cells detected by co-IP. k The luciferase activity of EphB2 measured by dual luciferase reporter system. WT: t = 8.236, P = 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Recombinant Mouse Ephb2 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+ephb2+protein/Ephrin-A1%2FEFNA1%2C+Mouse/pmc12983773-104-12-19
Average 94 stars, based on 1 article reviews

recombinant mouse ephb2 protein - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice"

Article Title: Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice

Journal: Cellular & Molecular Biology Letters

doi: 10.1186/s11658-026-00860-9

The activation of EFNB1/EphB2 interaction in dual-task intervention is regulated by miR-204. a Immunofluorescence of EFNB1 (red), EphB2 (green), NeuN (pink), with DAPI (blue). Scale bar 20 μm (Merge). b Quantification of EFNB1/EphB2 co-expression fluorescence intensity. F (4, 10) = 13.35, P < 0.001. c Expression of EFNB1 and EphB2 in the hippocampus detected by WB. d , e Quantification of EFNB1 and EphB2 expression in the hippocampus. EFNB1: H = 23.82, P < 0.001. EphB2: F (3, 26) = 18.69, P < 0.001. f EFNB1/EphB2 interactions in the hippocampus detected by co-IP. g Expression of EFNB1 and EphB2 in HT22 cells after silencing or overexpression lncRNA NEAT1/miR-204 detected by WB. h , i Quantification of EFNB1 and EphB2 expression in cells. EFNB1: F (4, 10) = 22.69, P < 0.001; EphB2: F (4, 10) = 18.13, P < 0.001 j EFNB1/EphB2 interactions in cells detected by co-IP. k The luciferase activity of EphB2 measured by dual luciferase reporter system. WT: t = 8.236, P = 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Figure Legend Snippet: The activation of EFNB1/EphB2 interaction in dual-task intervention is regulated by miR-204. a Immunofluorescence of EFNB1 (red), EphB2 (green), NeuN (pink), with DAPI (blue). Scale bar 20 μm (Merge). b Quantification of EFNB1/EphB2 co-expression fluorescence intensity. F (4, 10) = 13.35, P < 0.001. c Expression of EFNB1 and EphB2 in the hippocampus detected by WB. d , e Quantification of EFNB1 and EphB2 expression in the hippocampus. EFNB1: H = 23.82, P < 0.001. EphB2: F (3, 26) = 18.69, P < 0.001. f EFNB1/EphB2 interactions in the hippocampus detected by co-IP. g Expression of EFNB1 and EphB2 in HT22 cells after silencing or overexpression lncRNA NEAT1/miR-204 detected by WB. h , i Quantification of EFNB1 and EphB2 expression in cells. EFNB1: F (4, 10) = 22.69, P < 0.001; EphB2: F (4, 10) = 18.13, P < 0.001 j EFNB1/EphB2 interactions in cells detected by co-IP. k The luciferase activity of EphB2 measured by dual luciferase reporter system. WT: t = 8.236, P = 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Techniques Used: Activation Assay, Immunofluorescence, Expressing, Fluorescence, Co-Immunoprecipitation Assay, Over Expression, Luciferase, Activity Assay

EphB2 modulates the cAMP/PKA and PI3K/Akt pathways in the aging process. a Immunofluorescence for p16, 53BP1, and γH2AX in HT22 cells after EphB2 regulation. b – d Quantification of p16, 53BP1, and γH2AX fluorescence intensity in HT22 cells after EphB2 regulation. p16: F (2, 6) = 66.61, P < 0.001. 53BP1: F (2, 6) = 40.96, P < 0.001. γH2AX: F (2, 6) = 29.81, P < 0.001. e Expression of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 in cells detected by WB. f-o Quantification of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 levels. EFNB1: F (2, 6) = 53.83, P < 0.001. EphB2: F (2, 6) = 17.49, P = 0.003. cAMP: F (2, 6) = 70.05, P < 0.001. PKA: F (2, 6) = 50.13, P < 0.001. PI3K: F (2, 6) = 2.072, P = 0.207. Akt: F (2, 6) = 0.08, P = 0.924. p-PI3K: F (2, 6) = 55.45, P < 0.001. p-Akt: F (2, 6) = 17.91, P = 0.003. Surface NR1: F (2, 6) = 73.39, P < 0.001. Total NR1: F (2, 6) = 81.27, P < 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Figure Legend Snippet: EphB2 modulates the cAMP/PKA and PI3K/Akt pathways in the aging process. a Immunofluorescence for p16, 53BP1, and γH2AX in HT22 cells after EphB2 regulation. b – d Quantification of p16, 53BP1, and γH2AX fluorescence intensity in HT22 cells after EphB2 regulation. p16: F (2, 6) = 66.61, P < 0.001. 53BP1: F (2, 6) = 40.96, P < 0.001. γH2AX: F (2, 6) = 29.81, P < 0.001. e Expression of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 in cells detected by WB. f-o Quantification of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 levels. EFNB1: F (2, 6) = 53.83, P < 0.001. EphB2: F (2, 6) = 17.49, P = 0.003. cAMP: F (2, 6) = 70.05, P < 0.001. PKA: F (2, 6) = 50.13, P < 0.001. PI3K: F (2, 6) = 2.072, P = 0.207. Akt: F (2, 6) = 0.08, P = 0.924. p-PI3K: F (2, 6) = 55.45, P < 0.001. p-Akt: F (2, 6) = 17.91, P = 0.003. Surface NR1: F (2, 6) = 73.39, P < 0.001. Total NR1: F (2, 6) = 81.27, P < 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Techniques Used: Immunofluorescence, Fluorescence, Expressing

Concentration Assay:

Article Title: Cognitive-exercise dual-task training delays natural aging/D-galactose-induced cognitive decline in mice.
Article Snippet: .. Once the cells reached an appropriate concentration, they were exposed to either recombinant mouse EphB2 protein (HY-P75236, MCE, USA) or ALW-II-49-7 (HY-18833, MCE, USA) for a duration of 48 hours. ..

Article Title: Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice
Article Snippet: .. Once the cells reached an appropriate concentration, they were exposed to either recombinant mouse EphB2 protein (HY- P75236 , MCE, USA) or ALW-II-49-7 (HY-18833, MCE, USA) for a duration of 48 h. The control group received the same volume of vehicle. ..

Recombinant:

Control:

Image Search Results

Journal: Cellular & Molecular Biology Letters

Article Title: Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice

doi: 10.1186/s11658-026-00860-9

Figure Lengend Snippet: The activation of EFNB1/EphB2 interaction in dual-task intervention is regulated by miR-204. a Immunofluorescence of EFNB1 (red), EphB2 (green), NeuN (pink), with DAPI (blue). Scale bar 20 μm (Merge). b Quantification of EFNB1/EphB2 co-expression fluorescence intensity. F (4, 10) = 13.35, P < 0.001. c Expression of EFNB1 and EphB2 in the hippocampus detected by WB. d , e Quantification of EFNB1 and EphB2 expression in the hippocampus. EFNB1: H = 23.82, P < 0.001. EphB2: F (3, 26) = 18.69, P < 0.001. f EFNB1/EphB2 interactions in the hippocampus detected by co-IP. g Expression of EFNB1 and EphB2 in HT22 cells after silencing or overexpression lncRNA NEAT1/miR-204 detected by WB. h , i Quantification of EFNB1 and EphB2 expression in cells. EFNB1: F (4, 10) = 22.69, P < 0.001; EphB2: F (4, 10) = 18.13, P < 0.001 j EFNB1/EphB2 interactions in cells detected by co-IP. k The luciferase activity of EphB2 measured by dual luciferase reporter system. WT: t = 8.236, P = 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Article Snippet: Once the cells reached an appropriate concentration, they were exposed to either recombinant mouse EphB2 protein (HY- P75236 , MCE, USA) or ALW-II-49-7 (HY-18833, MCE, USA) for a duration of 48 h. The control group received the same volume of vehicle.

Techniques: Activation Assay, Immunofluorescence, Expressing, Fluorescence, Co-Immunoprecipitation Assay, Over Expression, Luciferase, Activity Assay

Journal: Cellular & Molecular Biology Letters

Article Title: Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice

doi: 10.1186/s11658-026-00860-9

Figure Lengend Snippet: EphB2 modulates the cAMP/PKA and PI3K/Akt pathways in the aging process. a Immunofluorescence for p16, 53BP1, and γH2AX in HT22 cells after EphB2 regulation. b – d Quantification of p16, 53BP1, and γH2AX fluorescence intensity in HT22 cells after EphB2 regulation. p16: F (2, 6) = 66.61, P < 0.001. 53BP1: F (2, 6) = 40.96, P < 0.001. γH2AX: F (2, 6) = 29.81, P < 0.001. e Expression of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 in cells detected by WB. f-o Quantification of EFNB1, EphB2, cAMP, PKA, PI3K, Akt, p-PI3K, p-Akt, total NR1, and surface NR1 levels. EFNB1: F (2, 6) = 53.83, P < 0.001. EphB2: F (2, 6) = 17.49, P = 0.003. cAMP: F (2, 6) = 70.05, P < 0.001. PKA: F (2, 6) = 50.13, P < 0.001. PI3K: F (2, 6) = 2.072, P = 0.207. Akt: F (2, 6) = 0.08, P = 0.924. p-PI3K: F (2, 6) = 55.45, P < 0.001. p-Akt: F (2, 6) = 17.91, P = 0.003. Surface NR1: F (2, 6) = 73.39, P < 0.001. Total NR1: F (2, 6) = 81.27, P < 0.001. All experiments were performed in at least three biological replicates. Values presented as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001

Techniques: Immunofluorescence, Fluorescence, Expressing

Maternal separation early in development causes visceral hyperalgesia in response to colorectal distension. (A) Schematic illustration of the experimental protocol. CRD, colorectal distension; MS, maternal separation (B) Score on abdominal withdrawal reflex after colorectal distension. Quantification is shown for eight animals per condition. CON, control. *P < 0.05, ****P < 0.0001 vs. CON, based on two-way repeated-measures ANOVA, followed by the Bonferroni multiple-comparisons test. (C) Representative thin sections of spinal cord after immunostaining against c-fos and quantification of cells expressing c-fos. CRD induced Fos protein expression in superficial lamine of spinal cord, we count the c-fos + neurons in lamine I-V of spinal cord of view at magnification ×10 and average the results. Results are shown for five animals per condition. Scale bar, 50 µm. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: **P < 0.01, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (D) Representative Western blotting of total lysate of spine tissue and quantification of EphB2 and EphrinB2. Protein levels were normalized to those of Actin in the same sample, and the relative protein level in the control group without CRD was defined as 1. Quantification is shown for four animals per treatment. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (E) Correlation between levels of c-fos and levels of EphB2 or EphrinB2 based on Western blotting of total lysates of spine tissue.

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Maternal separation early in development causes visceral hyperalgesia in response to colorectal distension. (A) Schematic illustration of the experimental protocol. CRD, colorectal distension; MS, maternal separation (B) Score on abdominal withdrawal reflex after colorectal distension. Quantification is shown for eight animals per condition. CON, control. *P < 0.05, ****P < 0.0001 vs. CON, based on two-way repeated-measures ANOVA, followed by the Bonferroni multiple-comparisons test. (C) Representative thin sections of spinal cord after immunostaining against c-fos and quantification of cells expressing c-fos. CRD induced Fos protein expression in superficial lamine of spinal cord, we count the c-fos + neurons in lamine I-V of spinal cord of view at magnification ×10 and average the results. Results are shown for five animals per condition. Scale bar, 50 µm. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: **P < 0.01, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (D) Representative Western blotting of total lysate of spine tissue and quantification of EphB2 and EphrinB2. Protein levels were normalized to those of Actin in the same sample, and the relative protein level in the control group without CRD was defined as 1. Quantification is shown for four animals per treatment. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (E) Correlation between levels of c-fos and levels of EphB2 or EphrinB2 based on Western blotting of total lysates of spine tissue.

Article Snippet: Mice that experienced maternal separation or not were anesthetized intraperitoneally using sodium pentobarbital (40 mg/kg), then injected intrathecally with one of the following: a chimera of EphB2 with Fc (catalog no. 467-B2, R&D Systems, Minnesota, United States), which binds to EphrinB2 and prevents it from stimulating endogenous EphB2; a chimera of EphrinB2 and Fc (catalog no. 496-EB, R&D Systems, Minnesota, United States), which binds and activates endogenous EphB2; or, as a negative control, recombinant Fc from human IgG1 (catalog no. 110-HG, R&D Systems, Minnesota, United States).

Techniques: Control, Immunostaining, Expressing, Western Blot

Visceral hyperalgesia in response to colorectal distension activates EphrinB2/EphB2 signaling and downstream MAP kinases. Mice were subjected to maternal separation (MS) early in development or not (CON), then later subjected to colorectal distension (CRD) or not. (A) Representative western blots of total lysate from spinal cord and quantification of the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK. Levels of phosphorylated protein were normalized to those of the corresponding total protein, and the relative level of phosphorylated protein in the control group without CRD was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (B–D) Immunostaining of thin sections of spinal cord against EphB2 (red) and the phosphorylated forms of p38, ERK, or JNK (green). Scale bar, 50 µm. The boxed regions in the large images are shown at higher magnification on the far right (“Zoom”). (E) Co-expression of spinal EphB2 and MAPKs. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ***P < 0.001 between CON and MS. (F) Proportion of spinal cord cells expressing each activated MAP kinase that also expressed EphB2 ( left plot ) or proportion of spinal cord cells expressing EphB2 that also expressed each of the phosphorylated MAP kinases ( right plot ).

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Visceral hyperalgesia in response to colorectal distension activates EphrinB2/EphB2 signaling and downstream MAP kinases. Mice were subjected to maternal separation (MS) early in development or not (CON), then later subjected to colorectal distension (CRD) or not. (A) Representative western blots of total lysate from spinal cord and quantification of the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK. Levels of phosphorylated protein were normalized to those of the corresponding total protein, and the relative level of phosphorylated protein in the control group without CRD was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ****P < 0.0001 between CON and CON + CRD or between MS and MS +CRD; #### P < 0.0001 between CON + CRD and MS + CRD. (B–D) Immunostaining of thin sections of spinal cord against EphB2 (red) and the phosphorylated forms of p38, ERK, or JNK (green). Scale bar, 50 µm. The boxed regions in the large images are shown at higher magnification on the far right (“Zoom”). (E) Co-expression of spinal EphB2 and MAPKs. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, ***P < 0.001 between CON and MS. (F) Proportion of spinal cord cells expressing each activated MAP kinase that also expressed EphB2 ( left plot ) or proportion of spinal cord cells expressing EphB2 that also expressed each of the phosphorylated MAP kinases ( right plot ).

Techniques: Western Blot, Control, Immunostaining, Expressing

Expression of EphrinB2 and EphB2 in neurons, astrocytes and microglia in spinal cord. (A, B) Mice that with (MS) or without (Con) experienced maternal separation were subjected to colorectal distension, then the spinal cord was thin-sectioned and immunostained against EphB2 (A) or EphrinB2 (B) and a marker for neurons (NeuN), activated microglia (Iba1), or astrocytes (GFAP). Scale bar, 50 µm. The boxed regions in the large images are shown at higher magnification on the far right (“Zoom”). (C, D) Co-expression of spinal EphB2 or EphrinB2 and NeuN, Iba1or GFAP. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, **P < 0.01 between CON and MS. (E, F) The proportion of EphB2 (E) or EphrinB2 (F) activation in neurons (NeuN), microglia (Iba1), or astrocytes (GFAP).

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Expression of EphrinB2 and EphB2 in neurons, astrocytes and microglia in spinal cord. (A, B) Mice that with (MS) or without (Con) experienced maternal separation were subjected to colorectal distension, then the spinal cord was thin-sectioned and immunostained against EphB2 (A) or EphrinB2 (B) and a marker for neurons (NeuN), activated microglia (Iba1), or astrocytes (GFAP). Scale bar, 50 µm. The boxed regions in the large images are shown at higher magnification on the far right (“Zoom”). (C, D) Co-expression of spinal EphB2 or EphrinB2 and NeuN, Iba1or GFAP. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, **P < 0.01 between CON and MS. (E, F) The proportion of EphB2 (E) or EphrinB2 (F) activation in neurons (NeuN), microglia (Iba1), or astrocytes (GFAP).

Techniques: Expressing, Marker, Activation Assay

Inhibition of EphrinB2/EphB2 signaling mitigates the effects of maternal separation. (A) Schematic illustration of the experimental protocol. Mice were subjected to maternal separation (MS) early in development or not (CON), intrathecally injected with a chimera of EphB2 and Fc (EphB2-Fc) that inhibits EphB2 signaling or a negative-control chimera (IgG-Fc), then subjected to colorectal distension (CRD), during which the abdominal withdrawal reflex (AWR) was measured. Subsequently, the spinal cord was analyzed by Western blotting and immunohistochemistry to observe expression of key proteins. (B) AWR score during CRD. Quantification is shown for eight animals per condition. Based on two-way repeated-measure ANOVA and the Bonferroni multiple-comparisons test: **P < 0.01, ****P < 0.0001 between CON + IgG-Fc and MS + IgG-Fc; # p < 0.05, ### p < 0.001 between MS + IgG-Fc and MS + EphB2-Fc. (C) Representative micrographs of spinal cord tissue after immunostaining against c-fos and quantification of the number of cells expressing c-fos. We count the c-fos + neurons in lamine I-V of spinal cord of view at magnification ×10 and average the results. Results are shown for five animals per condition. Scale bar, 50 µm. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: ****P < 0.0001 between MS + IgG-Fc and MS + IgG-Fc + CRD; ## P < 0.01 between MS + IgG-Fc + CRD and MS + EphB2-Fc + CRD. (D–F) Representative western blots of total lysate from spinal cord and quantification of (D) the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK; (E) the cell type markers NeuN, GFAP and Iba1; or (F) NR2B and NR2B phosphorylated on Tyr1472. Levels of phosphorylated protein were normalized to those of the corresponding total protein, while levels of markers, NR2B or phospho-NR2B were normalized to those of Actin; the resulting relative protein levels in the MS+IgG-Fc group were defined as 1. Quantification is shown for four animals per condition. Based on unpaired t-test, ****P < 0.0001 compared with MS + IgG-Fc group.

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Inhibition of EphrinB2/EphB2 signaling mitigates the effects of maternal separation. (A) Schematic illustration of the experimental protocol. Mice were subjected to maternal separation (MS) early in development or not (CON), intrathecally injected with a chimera of EphB2 and Fc (EphB2-Fc) that inhibits EphB2 signaling or a negative-control chimera (IgG-Fc), then subjected to colorectal distension (CRD), during which the abdominal withdrawal reflex (AWR) was measured. Subsequently, the spinal cord was analyzed by Western blotting and immunohistochemistry to observe expression of key proteins. (B) AWR score during CRD. Quantification is shown for eight animals per condition. Based on two-way repeated-measure ANOVA and the Bonferroni multiple-comparisons test: **P < 0.01, ****P < 0.0001 between CON + IgG-Fc and MS + IgG-Fc; # p < 0.05, ### p < 0.001 between MS + IgG-Fc and MS + EphB2-Fc. (C) Representative micrographs of spinal cord tissue after immunostaining against c-fos and quantification of the number of cells expressing c-fos. We count the c-fos + neurons in lamine I-V of spinal cord of view at magnification ×10 and average the results. Results are shown for five animals per condition. Scale bar, 50 µm. Based on one-way ANOVA and the Bonferroni multiple-comparisons test: ****P < 0.0001 between MS + IgG-Fc and MS + IgG-Fc + CRD; ## P < 0.01 between MS + IgG-Fc + CRD and MS + EphB2-Fc + CRD. (D–F) Representative western blots of total lysate from spinal cord and quantification of (D) the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK; (E) the cell type markers NeuN, GFAP and Iba1; or (F) NR2B and NR2B phosphorylated on Tyr1472. Levels of phosphorylated protein were normalized to those of the corresponding total protein, while levels of markers, NR2B or phospho-NR2B were normalized to those of Actin; the resulting relative protein levels in the MS+IgG-Fc group were defined as 1. Quantification is shown for four animals per condition. Based on unpaired t-test, ****P < 0.0001 compared with MS + IgG-Fc group.

Techniques: Inhibition, Injection, Negative Control, Western Blot, Immunohistochemistry, Expressing, Immunostaining

Activation of EphrinB2/EphB2 signaling reproduces the effects of maternal separation in naïve mice. (A) Schematic illustration of the experimental protocol. Mice that had not experienced maternal separation early in development were intrathecally injected with a chimera of EphrinB2 and Fc (EphB2-Fc) that activates EphB2 signaling or a negative-control chimera (IgG-Fc), then subjected to colorectal distension (CRD), during which the abdominal withdrawal reflex (AWR) was measured. Subsequently, the spinal cord was analyzed by Western blotting and immunohistochemistry to observe expression of key proteins. (B) AWR score during CRD. Quantification is shown for eight animals per condition. Based on two-way repeated-measure ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, **P < 0.01, ****P < 0.0001 vs IgG-Fc group. (C) Representative western blots of total lysate from spinal cord and quantification of the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK. Levels of phosphorylated protein were normalized to those of the corresponding total protein, and the relative level of phosphorylated protein in the IgG-Fc group was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and Bonferroni multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. IgG-Fc group, n = 4 mice in each group. (D, E) Representative western blots of total lysate from spinal cord and quantification of (D) the cell type markers NeuN, GFAP and Iba1; or (E) c-fos, NR2B, and NR2B phosphorylated on Tyr1472. Protein levels were normalized to those of Actin, and the relative protein level in the IgG-Fc group was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and Bonferroni multiple comparisons test, *P < 0.05, ***P < 0.001, ****P < 0.0001 vs. IgG-Fc group.

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Activation of EphrinB2/EphB2 signaling reproduces the effects of maternal separation in naïve mice. (A) Schematic illustration of the experimental protocol. Mice that had not experienced maternal separation early in development were intrathecally injected with a chimera of EphrinB2 and Fc (EphB2-Fc) that activates EphB2 signaling or a negative-control chimera (IgG-Fc), then subjected to colorectal distension (CRD), during which the abdominal withdrawal reflex (AWR) was measured. Subsequently, the spinal cord was analyzed by Western blotting and immunohistochemistry to observe expression of key proteins. (B) AWR score during CRD. Quantification is shown for eight animals per condition. Based on two-way repeated-measure ANOVA and the Bonferroni multiple-comparisons test: *P < 0.05, **P < 0.01, ****P < 0.0001 vs IgG-Fc group. (C) Representative western blots of total lysate from spinal cord and quantification of the active, phosphorylated forms of the MAP kinases ERK, p38 and JNK. Levels of phosphorylated protein were normalized to those of the corresponding total protein, and the relative level of phosphorylated protein in the IgG-Fc group was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and Bonferroni multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. IgG-Fc group, n = 4 mice in each group. (D, E) Representative western blots of total lysate from spinal cord and quantification of (D) the cell type markers NeuN, GFAP and Iba1; or (E) c-fos, NR2B, and NR2B phosphorylated on Tyr1472. Protein levels were normalized to those of Actin, and the relative protein level in the IgG-Fc group was defined as 1. Quantification is shown for four animals per condition. Based on one-way ANOVA and Bonferroni multiple comparisons test, *P < 0.05, ***P < 0.001, ****P < 0.0001 vs. IgG-Fc group.

Techniques: Activation Assay, Injection, Negative Control, Western Blot, Immunohistochemistry, Expressing

Proposed model for the role of EphrinB2/EphB2 signaling in visceral hyperalgesia due to stress in early life. Activation of spinal EphrinB2/EphB2 signaling alters glia-neuron interactions, which contributes to visceral hyperalgesia arising from the stress of earlier maternal separation. Upon activation of EphrinB2/EphB2 signaling, spinal astrocytes and microglia interact with neurons via synapses, activated glia release inflammatory mediators that induce excessive excitatory neuronal activity involving the NMDA receptor. Figure Created with BioRender.com .

Journal: Frontiers in Pharmacology

Article Title: Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation

doi: 10.3389/fphar.2024.1463339

Figure Lengend Snippet: Proposed model for the role of EphrinB2/EphB2 signaling in visceral hyperalgesia due to stress in early life. Activation of spinal EphrinB2/EphB2 signaling alters glia-neuron interactions, which contributes to visceral hyperalgesia arising from the stress of earlier maternal separation. Upon activation of EphrinB2/EphB2 signaling, spinal astrocytes and microglia interact with neurons via synapses, activated glia release inflammatory mediators that induce excessive excitatory neuronal activity involving the NMDA receptor. Figure Created with BioRender.com .

Techniques: Activation Assay, Activity Assay

Review

recombinant mouse ephb2 protein (MedChemExpress)

Structured Review

Images

1) Product Images from "Cognitive–exercise dual-task training delays natural aging/ d -galactose-induced cognitive decline in mice"

Related Articles

Concentration Assay:

Recombinant:

Control:

Similar Products

Image Search Results